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anti notch3 apc  (R&D Systems)


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    Structured Review

    R&D Systems anti notch3 apc
    Anti Notch3 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+notch3+apc/Human+Notch-3+APC-conjugated+Antibody/us10538587-2084-16-18
    Average 85 stars, based on 1 article reviews
    anti notch3 apc - by Bioz Stars, 2026-09
    85/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Antibodies against notch 3
    Article Snippet: Cell lines expressing mutant Notch3 and wild-type Notch3 (grown under standard conditions) were mixed with an anti-Notch3 binding and detection antibody that contains an APC fluorescein label (R&D cat# FAB1559A) in PBS containing 0.1% BSA and 0.01% sodium azide, and incubated for 1 hr at 4° C. After washing, the cells were analyzed by BD FACSCanto instrument using light and side scatter properties to gate on single cells. .. The level of Notch3 receptors on the cell surface was determined by binding of commercially available anti-Notch3 APC (R&D # FAB1559A) labeled antibody to cells expressing mutant and endogenous Notch3 and assessed by FACS. .. Cells were trypsinized (Invitrogen TrypLE cat#12605-010) and diluted to 2×106 cells/mL in FACS Buffer (PBS/3% FBS/0.01% NaN3).

    Labeling:

    Article Title: Antibodies against notch 3
    Article Snippet: Cell lines expressing mutant Notch3 and wild-type Notch3 (grown under standard conditions) were mixed with an anti-Notch3 binding and detection antibody that contains an APC fluorescein label (R&D cat# FAB1559A) in PBS containing 0.1% BSA and 0.01% sodium azide, and incubated for 1 hr at 4° C. After washing, the cells were analyzed by BD FACSCanto instrument using light and side scatter properties to gate on single cells. .. The level of Notch3 receptors on the cell surface was determined by binding of commercially available anti-Notch3 APC (R&D # FAB1559A) labeled antibody to cells expressing mutant and endogenous Notch3 and assessed by FACS. .. Cells were trypsinized (Invitrogen TrypLE cat#12605-010) and diluted to 2×106 cells/mL in FACS Buffer (PBS/3% FBS/0.01% NaN3).

    Expressing:

    Article Title: Antibodies against notch 3
    Article Snippet: Cell lines expressing mutant Notch3 and wild-type Notch3 (grown under standard conditions) were mixed with an anti-Notch3 binding and detection antibody that contains an APC fluorescein label (R&D cat# FAB1559A) in PBS containing 0.1% BSA and 0.01% sodium azide, and incubated for 1 hr at 4° C. After washing, the cells were analyzed by BD FACSCanto instrument using light and side scatter properties to gate on single cells. .. The level of Notch3 receptors on the cell surface was determined by binding of commercially available anti-Notch3 APC (R&D # FAB1559A) labeled antibody to cells expressing mutant and endogenous Notch3 and assessed by FACS. .. Cells were trypsinized (Invitrogen TrypLE cat#12605-010) and diluted to 2×106 cells/mL in FACS Buffer (PBS/3% FBS/0.01% NaN3).

    Mutagenesis:

    Article Title: Antibodies against notch 3
    Article Snippet: Cell lines expressing mutant Notch3 and wild-type Notch3 (grown under standard conditions) were mixed with an anti-Notch3 binding and detection antibody that contains an APC fluorescein label (R&D cat# FAB1559A) in PBS containing 0.1% BSA and 0.01% sodium azide, and incubated for 1 hr at 4° C. After washing, the cells were analyzed by BD FACSCanto instrument using light and side scatter properties to gate on single cells. .. The level of Notch3 receptors on the cell surface was determined by binding of commercially available anti-Notch3 APC (R&D # FAB1559A) labeled antibody to cells expressing mutant and endogenous Notch3 and assessed by FACS. .. Cells were trypsinized (Invitrogen TrypLE cat#12605-010) and diluted to 2×106 cells/mL in FACS Buffer (PBS/3% FBS/0.01% NaN3).

    FACS:

    Article Title: Antibodies against notch 3
    Article Snippet: Cell lines expressing mutant Notch3 and wild-type Notch3 (grown under standard conditions) were mixed with an anti-Notch3 binding and detection antibody that contains an APC fluorescein label (R&D cat# FAB1559A) in PBS containing 0.1% BSA and 0.01% sodium azide, and incubated for 1 hr at 4° C. After washing, the cells were analyzed by BD FACSCanto instrument using light and side scatter properties to gate on single cells. .. The level of Notch3 receptors on the cell surface was determined by binding of commercially available anti-Notch3 APC (R&D # FAB1559A) labeled antibody to cells expressing mutant and endogenous Notch3 and assessed by FACS. .. Cells were trypsinized (Invitrogen TrypLE cat#12605-010) and diluted to 2×106 cells/mL in FACS Buffer (PBS/3% FBS/0.01% NaN3).



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